Here you see the results (chromatogram) of the fatty acid analysis of the margarine. Which resolution problems are present in these data (zoom in on the middle part of the chromatogram)?

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additional files:

Not right. You can see in the chromatogram that the saturated and unsaturated fatty acids of the same chain length are well separated. If you zoom in on the peaks between 35 and 42 minutes you can see that all these peaks represent unsaturated fatty acids.

Not right. You can see in the chromatogram that the fatty acids with different chain length are completely separated. If you zoom in on the peaks between 35 and 42 minutes you can see that all these peaks represent fatty acids with 18 carbon atoms.

Not right. You can see in the chromatogram that the peaks between 35 and 42 minutes are not completely separated from each other. When you zoom in on this part you can see that all these peaks represent cis and trans isomers of mono unsaturated fatty acids with 18 carbon atoms. Some trans and cis isomers are not separated from each other.

Right!

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Poor resolution of saturated and unsaturated fatty acids of the same chain length.

Incomplete separation of cis and trans isomers

Coelution of FA with different chain length

Here you see the results (chromatogram) of the fatty acid analysis of the margarine. Study this chromatogram together with the data in the excel file.