The first step is: Hydrolysis of the protein molecules into their amino acids

To get trustworthy amino acids values, a complete hydrolysis is required without destruction or transformation of any particular amino acid. This is generally performed by acids hydrolysis of the peptide bonds (6M HCl, 105-120 0 C, 18-24hour). However this method has some drawbacks.

Do the exercises to learn about these drawbacks.