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Calculation of concentration of elements in a sample using AAS.

When a sample absorbs light, less light will reach the detector. See figure below. I o is the intensity of the light emitted by the hollow cathode lamp; I t, the transmitted light, is the intensity of the light after partial absorption of the light by the atoms in the flame. From the transmitted light the concentration of the element (c) can be calculated using the Lambert-Beer law.

a = molar extinction coefficient ; b= path length.