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Note that total fat can be accomplished in roughly two ways

• total fats in g per 100g edible portion (where total fat is determined by extraction of a food with an organic solvent (gravimetric procedures), the first part of this case). Most fat data in FCDB were realized in this way.

• total fatty acids expressed as triacylglycerol (TAG) equivalents: i.e. the amount of fatty acids converted to triglycerides. Chromatography analysis provides percentages of the different fatty acids classes in relation to the total fatty acids fraction (%FAME). This can be converted to absolute values (grams) by using an internal standard during the analysis of the sample. Usually a known amount of a fatty acid that normally is not present in the sample is added (e.g. C17 or C19 saturated fatty acid). The peak areas present in the chromatogram can be compared to the peak area of this internal standard. Because the amount of the internal standard is known, it is possible to calculate the amount of the other fatty acids. The latter approach is the recommended way of fat measurement

What do you expect, in general…

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Not right. When fat is extracted with a fat extraction method mentioned in the first part of the case also other components than fatty acids or triglycerides are extracted for example cholesterol, phospholipids etc.

Right. However, for margarine both methods will give almost the same values because fats in margarine consist for almost 100% of triglycerides.

Now you completed the FCDB and finished the part on fatty acids analysis!

…total fat (by extraction method) is higher than total fat expressed as TAG equivalents

…total fat (by extraction method) is lower than total fat expressed as TAG equivalents

…total fat (by extraction method) is equal to total fat expressed as TAG equivalents

|  | This is the information you would like to include in your database: Total fat (g/100g): 80 Total PUFA (g/100g) : 9.1 Total MUFA (g/100g) : 26.0 Total SFA (g/100g) : 29.8 Total trans fatty acids (g/100g) : 11.6 Total n-3 FA (g/100g) : 1.1 Total n-6 FA (g/100g): 8.0 |  |

Now we completed the whole table for you, but of course you can also try the calculations for n-6 and n-3 fatty acids yourself.